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Image Search Results


Simultaneous loss of ciliary ARL13B and PC1 rescues fibrosis and injury caused by loss of PC1 alone, as indicated by morphological ( a ), molecular ( b, c ), and cellular ( d, e ) analysis. ( a ) Sirius red and fast green staining reveals fibrosis in cyst-adjacent areas. ( b, c ) Western blot and densitometry analysis of the myofibroblast marker α-smooth muscle actin (α-SMA) in kidney lysates ( n = 3). ( d, e ) Quantification and images of renal injury marker SOX9 in kidney sections ( n = 5-6). Scale bars are 200 µm (a) and 50 µm (e). Genotypes in b-e correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.

Journal: bioRxiv

Article Title: Ciliary ARLs drive renal cystogenesis

doi: 10.1101/2025.11.20.689124

Figure Lengend Snippet: Simultaneous loss of ciliary ARL13B and PC1 rescues fibrosis and injury caused by loss of PC1 alone, as indicated by morphological ( a ), molecular ( b, c ), and cellular ( d, e ) analysis. ( a ) Sirius red and fast green staining reveals fibrosis in cyst-adjacent areas. ( b, c ) Western blot and densitometry analysis of the myofibroblast marker α-smooth muscle actin (α-SMA) in kidney lysates ( n = 3). ( d, e ) Quantification and images of renal injury marker SOX9 in kidney sections ( n = 5-6). Scale bars are 200 µm (a) and 50 µm (e). Genotypes in b-e correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.

Article Snippet: Densitometry analysis was performed with ImageLab software version 6.1 (Bio-Rad).

Techniques: Staining, Western Blot, Marker, Control

( a ) Western blot of Wnt signaling components β-catenin and cyclin D1 in kidney lysates. (b, c) Densitometry analysis of ( b ) β-catenin and ( c ) cyclin D1 ( n = 3). Genotypes in b, c correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.

Journal: bioRxiv

Article Title: Ciliary ARLs drive renal cystogenesis

doi: 10.1101/2025.11.20.689124

Figure Lengend Snippet: ( a ) Western blot of Wnt signaling components β-catenin and cyclin D1 in kidney lysates. (b, c) Densitometry analysis of ( b ) β-catenin and ( c ) cyclin D1 ( n = 3). Genotypes in b, c correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.

Article Snippet: Densitometry analysis was performed with ImageLab software version 6.1 (Bio-Rad).

Techniques: Western Blot, Control

Morphological, molecular, and cellular analysis reveal that ARL13B R79Q rescues fibrosis, injury, and Wnt signaling caused by loss of PC1. ( a ) Sirius red and fast green staining reveals fibrosis in cyst-adjacent areas. ( b, c ) Western blot and densitometry analysis of the myofibroblast marker α-SMA in kidney lysates ( n = 3). ( d, e ) Quantification and images of renal injury marker SOX9 in kidney sections ( n = 6). ( f ) Western blot of Wnt signaling components β-catenin and cyclin D1 in kidney lysates. ( g , h ) Densitometry analysis of ( g ) β-catenin and ( h ) cyclin D1 ( n = 3). Scale bars are 200 µm ( a ) and 50 µm ( e ). Genotypes in b-h correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.

Journal: bioRxiv

Article Title: Ciliary ARLs drive renal cystogenesis

doi: 10.1101/2025.11.20.689124

Figure Lengend Snippet: Morphological, molecular, and cellular analysis reveal that ARL13B R79Q rescues fibrosis, injury, and Wnt signaling caused by loss of PC1. ( a ) Sirius red and fast green staining reveals fibrosis in cyst-adjacent areas. ( b, c ) Western blot and densitometry analysis of the myofibroblast marker α-SMA in kidney lysates ( n = 3). ( d, e ) Quantification and images of renal injury marker SOX9 in kidney sections ( n = 6). ( f ) Western blot of Wnt signaling components β-catenin and cyclin D1 in kidney lysates. ( g , h ) Densitometry analysis of ( g ) β-catenin and ( h ) cyclin D1 ( n = 3). Scale bars are 200 µm ( a ) and 50 µm ( e ). Genotypes in b-h correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.

Article Snippet: Densitometry analysis was performed with ImageLab software version 6.1 (Bio-Rad).

Techniques: Staining, Western Blot, Marker, Control